SDLABIO Chamber Slide​

The steps of using a cell culture chamber, such as falcon chamber slides, may vary depending on the specific experimental purpose and sample type. The following are the general steps:
1. Preparation
Cleaning and Disinfection: Ensure that the cell culture slide chamber is clean and sterile before use. If it is a new product, it is usually pre – sterilized and can be used directly. If it is to be reused, first thoroughly clean it with a detergent to remove residual impurities, then rinse it several times with distilled water. After that, it can be sterilized by autoclaving or wiped with 75% alcohol, and then left to dry for use.
Prepare Experimental Materials: Prepare the required cells, culture medium, pipettes, pipette tips, sterile reagents, etc. Also, adjust the experimental instruments such as the laminar flow hood, centrifuge, and microscope to a normal working state.
2. Sample Addition
Cell Samples: If used for cell culture experiments in the cell culture slides, in the laminar flow hood, use a pipette to aspirate an appropriate amount of cell suspension in the logarithmic growth phase with good cell status, and slowly add it to each chamber of the cell culture chamber. Generally, determine the cell seeding density according to the size of the chamber and experimental requirements. For example, for a common 4 – well part of the cell culture chamber, the number of cells seeded per well is approximately (1×10⁴-1×10⁵). After seeding, gently transfer the cell culture chamber to the cell incubator and culture it under appropriate conditions (such as 37 Celsius, 5% CO2 to allow the cells to adhere and grow.
Tissue Samples: If observing tissue sections, first pre – treat the tissue samples by fixation, dehydration, embedding, etc., and make sections with an appropriate thickness (usually 4 – 10μm). Then, carefully place the sections in the chamber of the cell culture chamber using forceps, ensuring that the sections are flat. A small amount of adhesive (such as poly – L – lysine) can be used to help the sections adhere better to the slide.
3. Experimental Treatment
Drug Treatment: After the cells have adhered in the cell culture chamber, if conducting a drug experiment, according to the experimental design, use a pipette to add drug solutions of different concentrations to each chamber, and set up a control group without drugs at the same time. The volume of the drug needs to be determined according to the size of the chamber and experimental requirements, generally adding 100 – 500μL per well to achieve the preset concentration of the drug in the culture medium. After adding the drug, gently shake the cell culture chamber to evenly distribute the drug.
Staining Treatment: If conducting a staining experiment in the cell culture chamber, such as H&E staining of cells, first aspirate the culture medium in the chamber, and gently rinse the cells 2 – 3 times with PBS buffer to remove the residual culture medium. Then add hematoxylin stain, eosin stain, etc. in sequence for staining. The staining time is determined according to the stain instructions and experimental requirements, generally 3 – 5 minutes for hematoxylin staining and 1 – 3 minutes for eosin staining. After staining, rinse with running water to remove the excess stain.
4. Observation and Recording
Microscopic Observation: After the treatment is completed, place the cell chamber slides on the stage of the microscope. First, find the sample area under the low – power objective, and then switch to the high – power objective or oil – immersion objective for detailed observation. Pay attention to adjusting parameters such as focus, brightness, and contrast during observation to obtain a clear image. For example, when observing cell morphology in the cell culture chamber, features such as the cell outline, size, and the ratio of the nucleus to the cytoplasm can be observed; when observing tissue sections, the tissue layers and cell arrangements can be observed.
Image Recording: If it is necessary to record the experimental results of the microscope slide chamber, the imaging system by the microscope or a connected digital camera can be used for photographing. When photographing, select representative fields of view for shooting and record the shooting conditions (such as magnification, exposure time, etc.). For dynamic experiments, such as observing the cell movement process in the microscope chamber slides, time – lapse imaging technology can be used to take a photo at regular intervals to record the changes of cells over time.
5. Follow – up Treatment
Cleaning and Storage: If the cell culture chamber is to be reused, after the experiment, the samples and liquids in the chamber should be removed as soon as possible. Then, follow the cleaning and disinfection steps in the preparation to treat it, dry it, and store it properly.