SDLABIO Cell Culture Flask
SDLABIO Cell culture flask is a common laboratory appliance used for cell culture (culturing and propagating cells in vitro). It provides a controlled environment for cells, supports cell growth, division and metabolism, and is widely used in biomedical research, drug development, vaccine production and other fields.
Cell Culture Flask For Sale: T25, T75, T175, T225. Vented caps/ Sealed caps. TC treated optional.
MOQ: 1 CARTON.
Cell Culture Flask For Sale
| Cat No. | Dimendions | Package | Weight(kg) | Size(cm) |
| PYP-25-T | 25cm² filter cap, nontreated, sterile | 10 pcs/bag, 20 bags/ctn | 4.8 | 61*30*26 |
| PYP-75-T | 75cm² filter cap, nontreated, sterile | 5 pcs/bag, 20 bags/ctn | 9 | 49*43*38 |
| PYP-175-T | 175cm² filter cap, nontreated, sterile | 5 pcs/bag, 10 bags/ctn | 4.7 | 64*53*25 |
| PYP-225-T | 225cm² filter cap, nontreated, sterile | 5 pcs/bag, 5 bags/ctn | 3.8 | 78*27*27 |
| PYP-25-T-TC | 25cm² filter cap, TC treated, sterile | 10 pcs/bag, 20 bags/ctn | 4.8 | 61*30*26 |
| PYP-75-T-TC | 75cm² filter cap, TC treated, sterile | 5 pcs/bag, 20 bags/ctn | 9 | 49*43*38 |
| PYP-175-T-TC | 175cm² filter cap, TC treated, sterile | 5 pcs/bag, 10 bags/ctn | 4.7 | 64*53*25 |
| PYP-225-T-TC | 225cm² filter cap, TC treated, sterile | 5 pcs/bag, 5 bags/ctn | 3.8 | 78*27*27 |
| PYP-25 | 25cm² seal cap, nontreated, sterile | 10 pcs/bag, 20 bags/ctn | 4.8 | 61*30*26 |
| PYP-75 | 75cm² seal cap, nontreated, sterile | 5 pcs/bag, 20 bags/ctn | 9 | 49*43*38 |
| PYP-175 | 175cm² seal cap, nontreated, sterile | 5 pcs/bag, 10 bags/ctn | 4.7 | 64*53*25 |
| PYP-225 | 225cm² seal cap, nontreated, sterile | 5 pcs/bag, 5 bags/ctn | 3.8 | 78*27*27 |
| PYP-25-TC | 25cm² seal cap, TC treated, sterile | 10 pcs/bag, 20 bags/ctn | 4.8 | 61*30*26 |
| PYP-75-TC | 75cm² seal cap, TC treated, sterile | 5 pcs/bag, 20 bags/ctn | 9 | 49*43*38 |
| PYP-175-TC | 175cm² seal cap, TC treated, sterile | 5 pcs/bag, 10 bags/ctn | 4.7 | 64*53*25 |
| PYP-225-TC | 225cm² seal cap, TC treated, sterile | 5 pcs/bag, 5 bags/ctn | 3.8 | 78*27*27 |
Feature Of Cell Culture Flask
- Made of pure imported medical-grade polystyrene (PS), this material offers high transparency, enabling direct microscopic observation of cell growth.
- Optional cap types: Vent Cap: Built-in filter membrane to maintain gas exchange in a CO₂ incubator. Plug Seal Cap: Ideal for shipping or low gas exchange requirements.
- A variety of specifications are available: T25 cell culture flasks, T75 cell culture flask，cell culture flask t175，T225 cell flask.
- Surface modification options are available (TC-treated or non-TC-treated). TC-treated surfaces promote uniform attachment and growth of adherent cells. Non-TC-treated surfaces are suitable for suspended cells or specialized culture methods.
Flexible bottle neck design: Straight neck: Large opening for quick sample addition and liquid exchange. Angled neck: Convenient for microscopic observation and culture medium manipulation, reducing the risk of contamination.
FAQ of Cell culture flask
How many cells in t175 flask?
A T175 flask has approximately 175 cm² of growth area. The number of cells it can hold depends on the cell type and how dense you want them to grow.
For typical adherent mammalian cells, seeding densities usually range from 20,000 to 50,000 cells per cm². That means a fully confluent T175 flask can contain roughly 3.5 million to 8.75 million cells. For epithelial cells like HeLa, which are slightly denser, you might expect up to 10 million cells per flask. Stem cells, which grow more slowly and are seeded more sparsely, may reach 1.5 to 3.5 million cells per flask at full confluency.
Keep in mind that these numbers are approximate, as growth rate, passage number, and experimental conditions can affect the final cell count. Typically, a T175 flask is filled with 20–30 mL of culture medium to support healthy growth.
Why cell culture in different types of flask?
Tissue culture flasks come in different types because different experiments, cell lines, and workflows have different requirements for growth, observation, and handling. Here’s a clear breakdown:
1. Surface treatment (TC-treated vs. non-treated)
TC-treated flasks（adherent cell culture flask）:
- Surface modified to enhance cell adhesion.
- Used for adherent cells (e.g., fibroblasts, epithelial cells).
Non-treated flasks（suspension cell culture flask）:
- Smooth, hydrophobic surface that prevents attachment.
- Used for suspension cells or when you want to form spheroids.
2. Flask size (T25, T75, T175, T225.)
- Refers to the growth area in cm².
- Small flasks (t25 flask cell culture): for early-stage cultures, testing, or limited cell numbers.
- Larger flasks (T175): for producing bulk cells or scaling up.
- Choosing size helps balance incubator space, medium consumption, and yield.
3. Neck design (straight vs. angled)
Straight neck:
- Wider opening for easier pipetting and medium exchange.
Angled neck:
- Easier to reach cells with pipette and safer to observe under microscope without contamination risk.
4. Cap type (vented vs. sealed)
Cell culture flask vented cap:
- Allows gas exchange (CO₂ and O₂), suitable for standard incubator culture.
Sealed cap:
- Used for transport or when limited gas exchange is needed.
5. Material and color (standard clear vs. specialty flasks)
- Clear flasks: for direct microscopic observation.
- Opaque or coated flasks: for reducing light exposure or specialized applications.
Flask cell culture straight VS angle neck
1. Straight-Neck Flasks
- Features: Wide, vertical opening. Easier to insert pipettes or automated pipetting tips.
- Advantages: Fast medium exchange and cell seeding — less maneuvering. Good for routine work and automated systems.
- Limitations: More risk of contamination because the wide, vertical neck is fully open when uncapped. Observation under a microscope can be less convenient — you often have to tilt the flask.
2. Angle-Neck Flasks
- Features: Neck is slanted relative to the flask body. Opening is smaller and set at an angle.
- Advantages: Better control when adding/removing medium — reduces contamination risk. Easier microscopic observation — you can access the cell layer without tilting the flask.
- Limitations: Pipetting can be slightly slower or less ergonomic for large-volume liquid handling. May not fit as easily into some automated systems compared with straight-neck designs.
What are the differences between different sized cell culture bottles?
- 25cm2 cell culture flask: With a growth area of approximately 25 cm², it is suitable for small-scale cultures or initial experiments. Typically, 5–7 mL of culture medium is added. It is used for cryopreserved cell recovery, low-dose drug testing, or clone screening.
- 75cm2 cell culture flask: With a growth area of approximately 75 cm², it allows approximately three times the cell yield of a T25 flask. Typically, 10–15 mL of culture medium is added. It is suitable for routine experiments. It is used for routine cell maintenance and experiments; it is the most commonly used size.
- T175 tissue culture flask: With a growth area of approximately 175 cm², it allows for higher cell yields. Typically, 20–30 mL of culture medium is added. It is used for scale-up or harvesting large numbers of cells.
- T225 Cell Culture Flask: With a growth area of approximately 225 cm², it is suitable for large-scale cell preparation or experiments requiring a large number of starting cells. Typically, 30–40 mL of culture medium is added. The T175/T225 is suitable for large-scale cell preparation for protein expression, virus packaging, flow cytometry analysis, or animal experiment preparation.
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