SDLABIO Centrifuge Bottles

1. Applicable sample types and separation mechanisms

• Large volume cell processing
  Suspension cell harvesting: Separate adherent cells (such as HEK293) from the culture dish by low-speed centrifugation (such as 300-500 × g), and retain the cell pellet for subsequent passage or analysis.
  Cell debris removal: High-speed centrifugation (10,000 × g) breaks the cell lysate and collects soluble proteins or nucleic acids in the supernatant.
  Application case: In CAR-T cell therapy, centrifuge bottles are used to concentrate patient lymphocytes and remove culture medium impurities.
• Supernatant fine separation
  Layered extraction: Use density difference centrifugation (such as differential centrifugation) to separate the lipoprotein layer and the transparent layer in the serum.
  Virus purification: Ultracentrifugation (100,000 × g) concentrates lentiviral particles in the cell culture supernatant for gene therapy vector preparation.
• Microbial sample processing
  Bacterial/yeast culture: Centrifugation (5,000 × g) to collect logarithmic phase bacteria for plasmid extraction (such as E. coli) or metabolite analysis (such as yeast fermentation broth).
  Biofilm separation: Centrifugation removes planktonic bacteria in liquid culture medium and retains biofilm attached to the bottom of the centrifuge bottle for drug resistance research.
• Tissue sample processing
  Layering after homogenization: Centrifuge liver tissue homogenate (2,000 × g) to separate cell nuclei (precipitate) and mitochondria (supernatant requires further high-speed centrifugation).
  Tumor cell enrichment: Rare circulating tumor cells (CTCs) are isolated from tumor tissue by density gradient centrifugation (such as Percoll medium).

2. Key design to meet bioanalysis

• Chemical and biological compatibility
  Sterility certification: Gamma-sterilized disposable centrifuge bottles can be directly used for stem cell culture to avoid exogenous contamination.
  Corrosion-resistant material: Polypropylene (PP) centrifuge bottles can withstand strong denaturants such as proteinase K or TRIzol and are suitable for RNA extraction.
• Precise separation guarantee
  Scale accuracy: Centrifuge bottles with double scale lines (total volume and sediment volume) are convenient for directly reading the ratio of supernatant to sediment (such as ELISA sample pretreatment).
  Adaptive rotor design: Conical bottom centrifuge bottles match angle rotors to reduce centrifugal dead volume and improve the recovery rate of trace samples.

3.Specific applications in the field of life sciences

• Molecular biology
  Plasmid DNA preparation: Centrifuge to collect bacterial precipitates after alkaline lysis and remove protein impurities.
  CRISPR experiment: Centrifuge to remove cell fragments after genome editing and purify DNA required for editing efficiency detection.
• Cell biology
  Exosome separation: Exosomes are fractionated from conditioned culture medium by ultracentrifugation (100,000 × g).
  Subcellular component extraction: Differential centrifugation to separate organelles such as mitochondria and lysosomes.
• Synthetic biology
  Artificial cell construction: Centrifuge to remove unassembled lipid molecules in liposome synthesis and purify artificial membrane structures.