SDLABIO Glass Bottom Dish/Confocal Dish
SDLABIO Confocal dishes are designed for confocal microscopy and are used for real-time observation and analysis of living or fixed cells, ensuring a smooth transition from cell culture to cell analysis under the same seeding density, culture medium volume and culture conditions. It is suitable for high-resolution imaging, fluorescent labeling, and other research, and is widely used in cell biology, developmental biology and drug screening.
Confocal Culture Dish For Sale:
35mm confocal dish(Φ15mm/Φ20mm).
MOQ: 1 CARTON.
Confocal Dish For Sale
| Cat No. | Specification | Package | Weight(kg) | Size(cm) |
| GPYM-35-15 | 35mm, observation areaΦ15mm,sterilization,surface treatment,bagged | 10 pcs/bag, 20 bags/ctn | 2.2 | 30*23*19 |
| GPYM-35-20 | 35mm, observation areaΦ20mm,sterilization,surface treatment,bagged | 10 pcs/bag, 20 bags/ctn | 2.2 | 30*23*19 |
| GPYM-35-15-1 | 35mm, observation areaΦ15mm,sterilization,surface treatment,blister independent box | 20 pcs/box, 10 boxes/ctn | 2.2 | 30*23*19 |
| GPYM-35-20-1 | 35mm, observation areaΦ20mm,sterilization,surface treatment,blister independent box | 20 pcs/box, 10 boxes/ctn | 2.2 | 30*23*19 |
Feature Of Glass Bottom Dish
- High-performance glass substrate (No. 1.5 cover glass thickness, 160 μm ~190 μm), suitable for confocal and super-resolution microscopy.
- Strong cell compatibility: Optional TC (Tissue Culture) surface treatment promotes cell attachment and proliferation.
- No fluorescence background interference, suitable for fluorescent labeling experiments.
- Various sizes and formats: Common well diameters of 15 mm and 20 mm. Bags and boxes are available to meet diverse experimental needs.
- Compatible with mainstream microscopy systems: Suitable for confocal and fluorescence microscopes from brands such as Leica, Zeiss, Nikon, and Olympus.
- Standardized design: The glass bottomed dish size is compatible with automated pipetting and microscope stages; the cover plate features a vented design to maintain a stable cell culture environment.
FAQ Of Glass Bottom Dishes
How to use confocal microscopy dish?
- First, maintain a sterile operating environment when opening the package, typically in a clean bench or biosafety cabinet, to prevent dust or microbial contamination of the bottom of the culture dish. Before inoculating cells, cells need to be digested, centrifuged, and resuspended in a suitable culture medium from the culture flask or cell culture plate. Cells are then evenly added to the glass bottom cell culture dish at an appropriate density to ensure uniform cell distribution. This prevents observation difficulties caused by uneven cell density during microscopic imaging.
- During the incubation period, use a sterile pipette or pipette gun to gently change the culture medium, avoiding direct contact with the bottom of the dish to prevent scratching the glass or membrane. For extended experiments, it is recommended to use a constant temperature incubator to maintain stable temperature, humidity, and CO₂ concentration. If long-term live cell observation is required under a microscope, a constant temperature microscope observation device can be used.
There’s no need to transfer cells before imaging; simply place the confocal petri dish on the confocal microscope stage. Because the optical thickness of the bottom plate precisely matches the focal length of the confocal lens, it’s recommended to use dedicated lens oil or water immersion objectives, adjusting the focus and scanning parameters according to imaging requirements. During the experiment, syringe filters can be used to process the culture medium to ensure sterility, cell strainers can be used to remove cell clumps for improved imaging clarity, and serum bottles or culture medium bottles can be used to store and replenish fluids.
What is the difference between glass bottom culture dishes and regular cell culture dishes?
Confocal microscopy culture dishes use high-quality optical glass or film as their bottoms, ensuring clear imaging using laser confocal microscopy. Regular culture dishes, on the other hand, typically have thicker or less uniform substrates, making them unsuitable for high-resolution imaging.
Can confocal culture dish be used to culture cells long-term?
Absolutely. TC-treated glass bottom petri dishes, like standard culture dishes, support long-term adherent culture without the need for transferring cells prior to experimentation.
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