Sterile Petri Dishes
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SDLABIO Sterile Petri Dishes

SDLABIO Sterile petri dishes are laboratory consumables used in microbiology and cytology research. Made primarily of highly transparent polystyrene (PS), they undergo rigorous sterilization to ensure sterility. Widely used for bacterial and fungal culture, antimicrobial susceptibility testing, and cytology studies, these dishes are indispensable tools for basic experiments and scientific research.

Sterile Petri Dishes​ For Sale:

Round petri dish: 35mm/60mm/70mm/75mm/90mm/100mm/120mm/150mm. 90mm is available with two-division, three-division, or four-division grids.

Square petri dish: 100mm/130mm.

MOQ: 1 CARTON.  

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Sterile Petri Dishes For Sale

Cat No. Specification Package Weight(kg) Size(cm)
PYM-35 35mm, round 10 pcs/bag, 200bags/ctn 10.8 41*45*32
PYM-60 60mm, round 10 pcs/bag, 100bags/ctn 13.6 65*35*36
PYM-70 70mm, round 10 pcs/bag, 80bags/ctn 11.9 58*36*35
PYM-75 75mm, round, with ears 10 pcs/bag, 36bags/ctn 8.4 52*32*44
PYM-9015 90x15mm, round, 14.5g 10 pcs/bag, 50bags/ctn 10 49*49*36
PYM-9016 90x15mm, round, 16g 10 pcs/bag, 50bags/ctn 8.7 46.5*46.5*34.5
PYM-9020 90x20mm, round 10 pcs/bag, 50bags/ctn 8.7 49*49*42
PYM-902 90mm, round, two-part grid 10 pcs/bag, 50bags/ctn 11 49*49*36
PYM-903 90mm, round, three-part grid 10 pcs/bag, 50bags/ctn 11.6 49*49*36
PYM-904 90mm, round, four-part grid 10 pcs/bag, 50bags/ctn 12.3 49*49*36
PYM-100 100mm, round 10 pcs/bag, 32bags/ctn 7.1 43*44*38
PYM-120 120mm, round 10 pcs/bag, 24bags/ctn 7.5 52*38*47
PYM-150 150mm, round 10 pcs/bag, 20bags/ctn 4.15 48*32*30
PYM-15020 150x20mm, round 10 pcs/bag, 20bags/ctn 8.6 77*34*45
PYM-100F 100mm, square 10 pcs/bag, 40bags/ctn 7.1 57*46*39
PYM-130F 130mm, square 10 pcs/bag, 30bags/ctn 8.1 57*46*52

Feature Of Polystyrene Petri Dish

  • Made of pure imported medical-grade polystyrene (PS) with high transparency.
  • Smooth, flat surface, uniform thickness, and no optical distortion.
  • Stackable design saves space and facilitates storage.
  • Produced in a Class 100,000 cleanroom, it is DNA/RNA enzyme-free, pyrogen-free, and contains less than 0.1 EU/mL of endotoxin.
  • Available in a variety of sizes, small petri dish such as petri dish 35mm/60mm petri dish/70mm/75mm. Large petri dish such as 90mm petri dish/100mm petri dish/120mm/150mm petri dish,  including square petri dishes.

FAQ of plastic petri dishes

How to identify colonies on petrie dishes?

When observing microbial growth on a petry dish, colonies can usually be identified and differentiated by their morphological characteristics:

Size

  • Colonies may be pinpoint, small, medium, or large.
  • Bacterial species often show characteristic colony sizes under standard conditions.

Shape (Form)

  • Common forms include circular, irregular, filamentous, or rhizoid.

Edge (Margin)

  • Colony margins may be smooth (entire), undulate (wavy), lobate (lobed), curled, or filamentous.

Elevation

  • Colonies can appear flat, raised, convex, pulvinate (very convex), or umbonate (raised in the center).

Color and Pigmentation

  • Some colonies produce pigments (e.g., yellow, red, green).
  • Color can be inside the colony or diffused into the agar.

Surface and Texture

  • Colonies may be shiny, dull, smooth, rough, wrinkled, or mucoid (sticky and slimy).

Opacity

  • Colonies may be transparent, translucent, or opaque.

Hemolysis (on blood agar)

  • Some bacteria show alpha, beta, or gamma hemolysis, which helps in identification.

Growing bacteria in a microbes petri dish is a common laboratory practice for microbiology studies. Here are the basic steps:

  1. Prepare Nutrient Agar

    • Dissolve nutrient agar powder in distilled water and sterilize it (usually by autoclaving).

    • Pour the hot agar into sterile petri dish plate and let it solidify.

  2. Inoculate the Agar Surface

    • Use a sterile inoculating loop, needle, or cotton swab to transfer bacteria from a source (e.g., culture broth, environmental sample, or colony).

    • Gently streak or spread the bacteria across the agar surface.

  3. Seal and Incubate

    • Close the bacterial growth petri dish lid and seal it partially with parafilm or tape to reduce contamination while allowing some airflow.

    • Place the dish in an incubator set to the appropriate temperature (commonly 37°C for human pathogens, lower for environmental bacteria).

  4. Observe Growth

    • After 24–48 hours (or longer for slow-growing species), colonies should appear.

    • Examine the colonies based on their shape, size, color, and texture.

  5. Maintain Safety

    • Always practice aseptic techniques to avoid contamination.

    • Dispose of bacterial cultures properly by autoclaving or using disinfectants.

When grown in a petris dish, black mold (commonly Aspergillus niger or related species) typically shows these characteristics:

  1. Color

    • Colonies start out white or pale yellow and quickly turn dark brown to black as they mature.

    • The black color comes from dense spore production on the colony surface.

  2. Texture

    • Colonies often appear powdery, fuzzy, or velvety, due to the spore structures.

    • The center may be raised and darker, with lighter edges.

  3. Growth Pattern

    • Colonies usually spread outward in a circular pattern.

    • The edges can be slightly irregular or feathery.

  4. Underside of the Colony

    • The bottom (agar side) of the colony is often pale yellow, cream, or colorless, even if the top is black.

  5. Size and Speed

    • Black mold grows relatively quickly, covering large areas of the agar plate within a few days under warm, moist conditions.

Counting microbial colonies is a common task in microbiology to quantify bacterial growth. Follow these steps:

  1. Choose the Right Plate

    • Select a plate with 30–300 well-isolated colonies for accurate counting.

    • Plates with too many colonies may require dilution of the original sample.

  2. Prepare the Plate

    • Ensure the lab petri dish is on a flat, well-lit surface.

    • You can use a colony counter or grid to make counting easier.

  3. Count the Colonies

    • Count each visible colony individually.

    • Use a marker to tick off each colony on the plate bottom or on a grid overlay to avoid double counting.

    • For high-density plates, divide the plate into quadrants, count one quadrant, and multiply by four.

  4. Record and Calculate

    • Record the total colony count.

    • If the sample was diluted, calculate the original concentration using the dilution factor:

      CFU/mL=Volume plated (mL)Number of colonies×Dilution factor​​

  5. Tips for Accuracy

    • Use sterile technique to avoid contamination while handling the plate.

    • Always count colonies from the bottom of the laboratory petri dish, not the lid side.

    • For reproducibility, count each plate at least twice or have two people count independently.

  • Write on the bottom (agar side) – Always label the base of the microbiology petri dish, not the lid. The lid can be rotated or separated, but the bottom stays with your sample.
  • Use a permanent marker – Choose a fine-tip permanent marker that won’t smudge or wash off during incubation.
  • Include key information – Typically, you should write: sample name or ID number; date of inoculation; medium or experiment type; your initials (if needed).
  • Write around the edge – Place labels on the culture petri dish’s edge rather than in the center, so you can still observe colony growth clearly.
  • Keep it concise – Too much writing can block visibility. Use abbreviations if possible.

A disposable petri dish is a single-use container, usually made of polystyrene, designed for cultivating microorganisms. When you use it for bacteria petri dishes, you can conveniently grow and observe colonies without worrying about sterilization afterward. A petri culture dish, on the other hand, may be made of reusable glass or higher-quality materials and is commonly used in long-term laboratory research. Both are suitable as a bacterial culture petri dish, but disposable versions are more practical for routine experiments and teaching, while reusable ones are ideal for controlled, professional studies of petri dish bacteria.

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