SDLABIO Cell Culture Dish
SDLABIO Cell culture dishes are standardized laboratory consumables used for cell culture, cloning experiments, and microscopic observation. Available in multiple sizes to accommodate diverse experimental needs, they seamlessly integrate with microscopes, incubators, and automated pipetting equipment. They are a commonly used consumable in molecular biology, drug screening, stem cell, and tumor research.
Cell Culture Dishes For Sale:
35mm/60mm/100mm/150mm. TC treatment.
MOQ: 1 CARTON.
Cell Culture Dish For Sale
| Cat No. | Specification | Package | Weight(kg) | Size(cm) |
| PYM-35-TC-W | 35mm, with grip ring,sterile,TC | 20 pcs/bag, 25 bags/ctn | 2.2 | 30*22.5*25 |
| PYM-60-TC-W | 60mm, with grip ring,sterile,TC | 20 pcs/bag, 25 bags/ctn | 4.3 | 38*31*32 |
| PYM-100-TC-W | 100mm, with grip ring,sterile,TC | 10 pcs/bag, 30 bags/ctn | 8.7 | 62*52*24 |
| PYM-100-TC | 100mm, sterile,TC | 10 pcs/bag, 30 bags/ctn | 7.9 | 66*56*25 |
| PYM-150-TC-W | 150mm, with grip ring,sterile,TC | 5 pcs/bag, 12 bags/ctn | 4.15 | 48*32*30 |
Feature Of Microbiology Petri Dish
- Made of pure imported medical-grade polystyrene (PS) with high transparency.
- The surface is smooth and flat, distortion-free, with uniform thickness and no optical distortion.
- The stackable design saves space and facilitates storage.
- The cell culture dishes are TC-treated for excellent adherence.
- Produced in a Class 100,000 cleanroom, they are DNA/RNA enzyme-free, pyrogen-free, and contain less than 0.1 EU/mL of endotoxin.
- Available in a variety of sizes: 35mm, 60mm, 100 mm, 150mm cell culture dish. 100mm petri dishes are also available with a non-grip ring.
FAQ Of Petri dish for cell culture
How to choose a cell culture dish with different diameters?
- Petri dish 35mm: Suitable for small-scale experiments, transfection tests, or cloning experiments.
- 60mm petri dish/100 mm petri dish: Suitable for medium-scale cell expansion or drug treatment experiments.
- 15cm dish cell culture: Suitable for large-scale cell expansion or protein and virus production.
What is the difference between TC-treated and untreated culture dishes?
- TC-treated dishes: Suitable for most adherent cells, significantly improving cell attachment and growth.
- Untreated dishes: Suitable for suspension cell culture or experiments requiring cell adhesion prevention (e.g., low-attachment screening).
Can cell culture dishes be used for extended periods?
Absolutely, but care must be taken to regularly change the culture medium and maintain sterile procedures. For live cell imaging, glass-bottom confocal culture dishes or use with a constant-temperature microscope are recommended.
How are cell culture dishes used?
1. Preparation
- Work in a clean bench or biosafety cabinet to ensure a sterile environment.
- Prepare the cell suspension, culture medium, pipette, sterile pipettes or tips, and other related consumables.
- Inspect the sterile petri dishes packaging for integrity and no damage, and verify sterility.
2. Seeding Cells
- When opening the culture dish, avoid direct contact between the lid and the work surface or hands to prevent contamination.
- Gently add an appropriate amount of cell suspension to the center of the culture dish. Then gently shake or rotate the dish crosswise to ensure that the cells are evenly distributed across the bottom of the dish.
- Choose an appropriate seeding density: too low a density may affect growth rate, while too high a density may lead to over-confluence.
3. Culture and Maintenance
- Place the cell culture dish in a constant temperature incubator (typically 37°C, 5% CO₂). The volume of culture medium should be sufficient to cover the bottom of the cells, but not excessive to prevent gas exchange.
- When changing the culture medium or adding drugs, be gentle to avoid scraping the bottom of the dish or dispersing the cells. Serum bottles (square culture medium bottles) can be used to provide fresh culture medium and sterilize it through a syringe filter.
- For extended culture periods, regularly monitor the cell status and adjust the culture medium as needed.
4. Microscope Observation and Experimental Procedures
- Highly transparent or glass-bottomed cell culture dishes can be used directly for observation under inverted, phase-contrast, fluorescence, and confocal microscopes without transferring cells.
- If cells need to be dissociated, trypsin or a cell scraper can be used to detach them from the bottom of the dish and then transferred to other consumables such as cell culture flasks or centrifuge tubes for subsequent experiments.
5. Precautions
- Maintain aseptic technique throughout the entire process and avoid contact with the inner surface of the culture petri dish.
- Polystyrene petri dishes are typically disposable and should not be reused to prevent scratches or residue from affecting cell growth and experimental results.
- When handling culture medium or discarded cells, collect them in biological specimen bags and adhere to biosafety regulations.
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