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SDLABIO Spin Columns for DNA and RNA Extraction and Purification

SDLABIO Spin columns are designed for the purification of DNA, RNA, plasmids, PCR products, and viral nucleic acids. Equipped with high-quality silica membranes or glass fiber membranes, these columns ensure efficient binding and reliable elution. The column tube and collection tube are made from medical-grade polypropylene, offering excellent chemical resistance and compatibility with commonly used lysis buffers and wash solutions.

Spin Column For Sale:
4-layer/6-layer/8-layer/10-layer/12-layer silicone film.

MOQ: 1 CARTON.  

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Spin Column for Sale

Cat No. Specification Packaging
CHZ-01-4 Outer tube without cap, inner tube with cap, 4-layer filter membrane, blue retaining ring 100pcs/bag, 50 bags/ctn
CHZ-01-6 Outer tube without cap, inner tube with cap, 6-layer filter membrane, blue retaining ring 100pcs/bag, 50 bags/ctn
CHZ-01-8 Outer tube without cap, inner tube with cap, 8-layer filter membrane, blue retaining ring 100pcs/bag, 50 bags/ctn
CHZ-02-4 Outer tube without cap, inner tube with cap, 4-layer filter membrane, transparent retaining ring 100pcs/bag, 50 bags/ctn
CHZ-02-6 Outer tube without cap, inner tube with cap, 6-layer filter membrane, transparent retaining ring 100pcs/bag, 50 bags/ctn
CHZ-02-8 Outer tube without cap, inner tube with cap, 8-layer filter membrane, transparent retaining ring 100pcs/bag, 50 bags/ctn
CHZ-03-4 Outer tube with cap, inner tube without cap, 4-layer filter membrane, transparent retaining ring. 100pcs/bag, 50 bags/ctn
CHZ-03-6 Outer tube with cap, inner tube without cap, 6-layer filter membrane, transparent retaining ring. 100pcs/bag, 50 bags/ctn
CHZ-03-8 Outer tube with cap, inner tube without cap, 8-layer filter membrane, transparent retaining ring. 100pcs/bag, 50 bags/ctn

Feature Of Spin Columns

  • The column tubes are injection molded from medical-grade imported polypropylene and assembled in a cleanroom automated production line, ensuring ultra-pure product.
  • High-quality silicone membranes provide stable performance, minimal intra-batch and inter-batch variation, and excellent repeatability.
  • Optimized column design minimizes dead volume, effectively preventing liquid retention and salt residue.
  • High specificity, resistant to prolonged high-speed centrifugation and multiple solution processing.
  • High purity and high extraction yield.
  • The product is available in aseptic sealed packaging, is ultra-pure, free of DNase/RNase, PCR inhibitors, pyrogens, and endotoxins.

FAQ Of Spin Columns For DNA Purification

Can purification columns be reused?

No. Spin columns are single-use consumables; reuse may cause contamination and reduced binding capacity.

SDLABIO spin columns withstand 12000×g, suitable for standard high-speed centrifuges.

A spin column contains a silica or fiber membrane for selective nucleic acid binding, while a regular centrifuge tube only holds liquid without purification capability.

1. Structure of a Column Purification:

A spin column consists of:

  • A plastic column (usually made of polypropylene or another durable material)
  • A membrane inside the column, often made of silica gel or glass fiber. This membrane is the key component for binding nucleic acids.
  • A collection tube that collects the flow-through (unbound solution) and eluate (purified sample).

2. Basic Principle:

Spin columns rely on centrifugation to create a force that helps separate particles or molecules. The core principle is that nucleic acids (DNA or RNA) are selectively bound to the membrane in the column, and then impurities are washed away. The nucleic acids are then eluted (released) in a pure form.

3. The Step-by-Step Process:

Step 1: Lysis of the Sample

  • First, a sample containing nucleic acids (such as blood, tissues, cells, or bacteria) is mixed with a lysis buffer. The buffer breaks open the cells and releases the DNA or RNA into the solution.

Step 2: Binding to the Membrane

  • The lysed sample is then applied to the spin column. The silica membrane inside the column has a unique property: it binds nucleic acids (DNA, RNA) in the presence of high-salt conditions.
  • The sample is centrifuged at a high speed (usually around 10,000 to 15,000 x g). This generates a centrifugal force that forces the liquid through the column and into the collection tube.

  • Nucleic acids will bind to the membrane, while impurities (proteins, salts, and other contaminants) will pass through and be discarded in the waste liquid.

Step 3: Washing

  • The next step involves adding a wash buffer to the DNA spin column purification. The wash buffer helps to remove any remaining impurities (such as salts, proteins, and small molecules) from the membrane.

  • The column is spun again to remove this wash solution, leaving the nucleic acids bound to the membrane.

Step 4: Elution

  • After washing, a low-salt buffer (or water) is added to the column. This buffer helps to release (elute) the purified nucleic acids from the membrane.

  • The column is spun once more to collect the purified DNA or RNA in the collection tube.

4. Why Does It Work?

  • Selective Binding: Silica membranes are designed to selectively bind nucleic acids in the presence of high salt. Under these conditions, the negative charges on the nucleic acids interact with the silica surface.

  • Centrifugation: Centrifugation is the critical force that separates the sample components by mass. Nucleic acids are retained on the membrane, while smaller impurities are removed with the washing buffer.

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