SDLABIO Ultrafiltration Centrifuge Tube
SDLABIO Ultrafiltration centrifuge tubes are laboratory consumables used for sample concentration, desalting, and buffer exchange. They are suitable for processing proteins, nucleic acids, and peptides. Equipped with ultrafiltration membranes of different MWCO options, the tubes efficiently retain target molecules while removing small molecular impurities, and are widely used in molecular biology, proteomics, and clinical testing applications.
Ultrafiltration Tube For Sale:
15ml/50ml;PES ultrafiltration membranes (MWCO: 5 kDa, 10 kDa, 30 kDa, 50 kDa, and 100 kDa) and PES microfiltration membranes (pore sizes: 0.1 μm, 0.2 μm, and 0.5 μm) are available.
MOQ: 1 CARTON.
Ultrafiltration centrifuge tube for sale
| Cat No. | Specification | Centrifuge Type |
| UT-04005-PES | Inner tube 4ml_PES ultrafiltration membrane_5kd | Centrifuge for standard 15ml conical tubes |
| UT-04010-PES | Inner tube 4ml_PES ultrafiltration membrane_10kd | |
| UT-04030-PES | Inner tube 4ml_PES ultrafiltration membrane_30kd | |
| UT-04050-PES | Inner tube 4ml_PES ultrafiltration membrane_50kd | |
| UT-04100-PES | Inner tube 4ml_PES ultrafiltration membrane_100kd | |
| MT-0401-PES | Inner tube 4ml_PES microfiltration membrane_0.1μm | |
| MT-0402-PES | Inner tube 4ml_PES microfiltration membrane_0.2μm | |
| MT-0405-PES | Inner tube 4ml_PES microfiltration membrane_0.5μm | |
| UT-15005-PES | Inner tube 15ml_PES ultrafiltration membrane_5kd | Centrifuge for standard 50ml conical tubes |
| UT-15010-PES | Inner tube 15ml_PES ultrafiltration membrane_10kd | |
| UT-04030-PES | Inner tube 15ml_PES ultrafiltration membrane_30kd | |
| UT-04050-PES | Inner tube 15ml_PES ultrafiltration membrane_50kd | |
| UT-04100-PES | Inner tube 15ml_PES ultrafiltration membrane_100kd | |
| MT-0401-PES | Inner tube 15ml_PES microfiltration membrane_0.1μm | |
| MT-0402-PES | Inner tube 15ml_PES microfiltration membrane_0.2μm | |
| MT-0405-PES | Inner tube 15ml_PES microfiltration membrane_0.5μm |
Feature of ultrafiltration tubes
- Material: Filter membrane material: polyethersulfone (PES), tube material: polypropylene (PP), tube cover material: high-density polyethylene (HDPE).
- Low dead-volume design minimizes sample loss during centrifugation.
- Protein recovery efficiency is as high as 85% or above, with excellent reproducibility.
- The tube body features precise black graduation markings, along with MWCO labeling and a writable white area for easy identification and labeling.
- High-efficiency retention: Available in a variety of molecular weight cut-off options (e.g., 5 kDa, 10 kDa, 30 kDa, 50 kDa, and 100 kDa) to meet the processing needs of different biomolecules.
- Low biomolecule binding: Biomolecules are not easily adsorbed by the filtration membrane, reducing the risk of denaturation or loss of activity.
- High chemical compatibility: Suitable for a wide range of experimental conditions and resistant to various chemical reagents.
- Applicable for the concentration, desalting, and purification of dilute solutions or purified proteins (such as chromatography eluates) of proteins, antigens, antibodies, enzymes, viruses, and microorganisms.
FAQ Of ultrafiltrate tube
What's the difference between ultrafilter centrifuge tubes and standard centrifuge tubes?
Standard centrifuge tubes are primarily used for sample sedimentation and separation, while ultrafiltration centrifuge tubes incorporate an ultrafiltration membrane within the tube, which selectively traps molecules. These tubes are commonly used for sample concentration and desalting, providing a more refined separation tool in molecular experiments.
How do I choose the right molecular weight cutoff (MWCO)?
Generally, you should choose a MWCO that’s 2–6 times lower than the molecular weight of your target molecule. For example, for a protein with a molecular weight of 50 kd, a 30 kda centrifugal filter tube is recommended to ensure effective retention.
Do centrifuge filter tube require a special centrifuge?
No, a regular benchtop high-speed centrifuge will work. Simply follow the instructions to select the appropriate speed and time.
How to use centrifuge filter tubes?
Centrifuge filter tubes (ultrafiltration tubes) are simple to use and are mainly used for sample concentration, desalting, and buffer exchange. The general workflow is as follows:
- Pre-rinse (if required): Some membranes may require pre-wetting or rinsing with buffer or ultrapure water to improve performance and reduce initial sample loss.
- Add sample: Transfer your protein, nucleic acid, or other solution into the upper chamber of the filter tube. Do not exceed the maximum volume mark.
- Balance the tubes: Place the filter tube in a centrifuge alongside a counterbalance tube of equal weight to ensure safe operation.
- Centrifugation: Set the appropriate speed and time according to the product instructions (typically within the recommended RCF range). The solvent and small molecules pass through the membrane, while target molecules are retained.
- Check concentration level: After centrifugation, check the retentate volume in the upper chamber. Repeat centrifugation if further concentration is needed.
- Sample recovery: Carefully pipette out the concentrated sample from the upper chamber.
- Optional washing (for desalting/buffer exchange): Add fresh buffer and repeat centrifugation to remove unwanted small molecules.
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