PCR (polymerase chain reaction) is a technique used to amplify specific DNA fragments. It quickly replicates the target DNA sequence in vitro by simulating the natural replication process of DNA in cells. The preparation before the PCR reaction is the key to the success of the experiment. Only by ensuring the quality of template DNA, reasonable primer design, accurate reaction system, correct instrument settings and standardized operation can reliable and efficient PCR results be obtained. Ignoring any step may lead to experimental failure or inaccurate results.


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